Students will genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance. They will then examine the number of resistant bacteria to determine the efficiency of the transformation procedure. Teachers manual and Student Study Guide copymasters are included. Materials provided for 8 lab groups. Meets AP Science Practices 1, 3, 5, 6, and 7, and Big Idea 3.

Kit Includes:
30mL Sterile Calcium Chloride 0.05M
16 Sterile Volumetric Pipet
8 Pipet Bulb
8 Micropipet w/Plunger, 10ul
1 Plastic Centrifuge Tube
16 Microcentrifuge Tube, 1.5mL
8 Sterile Inoculating Loop
40 Petri Dish
4 Luria Agar, 200mL
1 E. Coli Culture (freeze dried)
1 Luria Broth, 9mL Tube

Kit Contains Coupon for Following Perishable Materials: (Redeem by fax, phone or e-mail) 
2 btls 0.02g Ampicillin
1000 ng 1 pUC19 Plasmid
1 Tryptic Soy Broth Tube, 9mL
1 Tryptic Soy Agar Slants, 6mL
1 Pipet, Sterile, Disposable
1 pkg Cotton Swabs, Sterile

Materials Needed But Not Supplied:
Large beaker
Hot plate or waterbath
Microwave (not necessary if melting agar in a waterbath)
Markers
Microfuge tube racks
Container of ice
Incubator (37°C) recommended