While over 99% of DNA is the same among all humans, the remaining part is unique to every individual (with the exception of identical twins). These differences are hereditary, with parts of the unique sequence coming from each parent. By using DNA fingerprinting, or DNA profiling, a child’s paternal relationship can be tested by comparing the child’s DNA profile to the profile of the mother and possible father to determine if an individual is the father or not. In this activity students will use electrophoresis to separate DNA samples of a child, mother, and two potential fathers to determine if either father is a possible paternal match.

**Not included but required are agarose electrophoresis chambers, electrophoresis power supplies and micropipettes capable of measuring 10µL.**

Kit Includes:

200mL Prepared Agarose 0.8% Solution
500mL Tris Borate EDTA Buffer 5X
60mL DNA Stain
10μg Mother’s DNA Sample
10μg Child’s DNA Sample
10μg Possible Father #1 DNA Sample
10μg Possible Father #2 DNA Sample